Hematological effects of lead exposure and Moringa intervention in rats
DOI:
https://doi.org/10.5281/zenodo.13772448Keywords:
lead acetate, defatted Moringa oleifera seed meal, red blood cell, white blood cellAbstract
In this study, the hematological effects of lead acetate and defatted Moringa oleifera seed meal (DMOSM) were investigated in male Wistar rats. Eighty Wistar rats were assigned into 5 groups (16 rats each) as follows: Group I (negative control), II (lead acetate solution at 480 mg/kg), III (lead acetate solution and DMOSM at 480 mg/kg each, simultaneously), IV (lead acetate solution at 480 mg/kg for the first 14 days, followed by DMOSM at 480 mg/kg for the next 14 days, and V (DMOSM at 480mg/kg). Treatments were administered orally and daily for 28 days. Blood was collected on days 7, 14, 21, and 28 and processed for hematological examination. Results revealed non-significant (p > 0.05) increases in red blood cell parameters (PCV, Hb, RBC) at 7, 14, 21, and 28 days in groups II, III, IV, and V compared to the negative control (Group I). Group III exhibited the highest values, suggesting a potential compensatory mechanism against lead-induced anemia. White blood cell indices showed non-significant (p > 0.05) fluctuations, with Group III displaying a persistent immunomodulatory effect. Neutrophil counts were significantly (p < 0.05) higher in all treatment groups at 7 days, indicating a potential early inflammatory response. In conclusion, the results suggest a potential ameliorative effect of DMOSM on lead-induced haematological alterations. The study, therefore, highlights the intricate interplay between lead toxicity and the protective effects of M. oleifera, underscoring the need for further research to elucidate the underlying mechanisms and validate these findings in diverse populations.
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