Monitoring of Aflatoxins in Broiler, Quail and Ostrich feed samples


  • Roheela Yasmeen Department of Biology, Lahore Garrison University, Lahore
  • Aamir Hussain Department of Biology, Lahore Garrison University, Lahore
  • Faheem Hafeez Department of Biology, Lahore Garrison University, Lahore
  • Khalid Saeed Food and Biotechnology Research Centre, PCSIR, Laboratories Complex, Ferozepur Road, Lahore



Aflatoxin, Detoxification,, Quail,, Contamination, Broiler, Ostrich


Aflatoxins are types of biological toxins also known as mycotoxins and can be produced by 100 different types of fungi. The most common types of mycotoxins are AFB1, AFB2, AFG1, and AFG2. These are toxic metabolites that are produced by Aspergillus flavus and Aspergillus parasiticus. In the present study, the most common method thin layer chromatography (TLC) was used to estimate the levels of aflatoxins in broiler, quail, and ostrich feed samples. A total of 18 samples were collected from different sites of broiler, quail, and ostrich farms and local shops in Lahore. Upon quantification by TLC method, the contamination was detected in 12 samples (66.66%) out of 18 samples. B1, B2, and G1 aflatoxins were detected in different feed samples. The prevalence of the aflatoxins in overall feed samples was noticed in the following order B1>B2>G1. A less amount of contamination was noticed in broiler feed samples as compared to quail and ostrich feed samples. Results were statistically analyzed by one-way ANOVA and significant differences at p-value < 0.05 were noticed for aflatoxins in broilers with quail and ostrich. While non-significant differences at p-value> 0.05 were noticed for ostrich and quail feed samples. By independent sample T-test, a significant difference was noticed in positive feed samples of summer and winter seasons at p-value < 0.05.  The most contaminated samples were found in the summer season as compared to the winter season. However, it was concluded that might be the storage conditions of feed samples need to improve and should be regulated by feed authorities so that the effects of aflatoxins can be reduced in the feed samples.


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How to Cite

Yasmeen, R., Hussain, A., Hafeez, F., & Saeed, K. . (2023). Monitoring of Aflatoxins in Broiler, Quail and Ostrich feed samples. Scientific Reports in Life Sciences, 4(2), 11–23.